Install instructions for hmm script on Ubuntu or Debian systens 1 - install the ghmm package 1.1 Install depending packages (see www.ghmm.org for details) sudo apt-get install debhelper autotools-dev libglib2.0-0 libgl1-mesa-glx mime-support libmagic1 locales file libxml2-dev libasound2 lib32asound2 pkg-config automake libpcre3 gawk libtool 1.2 Download ghmm from http://www.cin.ufpe.br/~igcf/Metabolites/scripts/hmm/ghmm0.8.zip (for 64 bits) or http://www.cin.ufpe.br/~igcf/Metabolites/scripts/hmm/ghmm.tar.gz (for 32 bits) 1.3 Extract file, go into ghmm directory and run commands ./autogen.sh ./configure make sudo make install 2 - Instal Pymix 2.1 Install depending packages (see www.pymix.org for details) sudo apt-get install python-dev python-numpy libgsl0-dev 2.2 Download pymix from http://www.cin.ufpe.br/~igcf/Metabolites/scripts/hmm/pymix-0.8a.zip 2.3 Extract file, go into pymix directory and run commands python setup.py build sudo python setup.py install 3 Install GQL 3.1 Install depending packages sudo apt-get install python-numpy python-scipy 2.2 Download GQL from http://www.cin.ufpe.br/~igcf/Metabolites/scripts/hmm/GQL2.1.zip 2.3 Extract file, go into pymix directory and run commands python setup.py build sudo python setup.py install 4 Download http://www.cin.ufpe.br/~igcf/Metabolites/scripts/hmm/train_rank.py and use it to run experiments(see train_rank.py for options) - For example to run the HMM with 2 states on co2 data (obtained at http://www.cin.ufpe.br/~igcf/Metabolites/data/Biological%20Data%20Python/) run python train_rank.py -s 2 arab_met_co2.txt arab_co2.txt arab_co2_hmm2 - Results files arab_co2_hmm2.res contains the likelihood of genes (lines) vs. metabolites (collumns).